Epigenetics & Nuclear Signaling Antibodies 1

Kaiso/ZBTB33 Rabbit Polyclonal Antibody (CAB12900)

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SKU:
CAB12900
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
ZNF348, ZNF-kaiso, Kaiso/ZBTB33
Research Use:
Epigenetics and Nuclear Signaling
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Description

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Kaiso/ZBTB33 Antibody (CAB12900)

The Kaiso/ZBTB33 Antibody (CAB12900) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.ZBTB33, also known as Kaiso, is a transcription factor that interacts with specific DNA sequences to regulate gene expression. Its involvement in diverse cellular functions, including cell proliferation, differentiation, and apoptosis, makes it a compelling target for investigations in various fields such as molecular biology, developmental biology, and cancer research.

This antibody is validated for use in WB, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Kaiso/ZBTB33 Antibody
SKU:CAB12900
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PCRY CEKV FPLA EYRT KHEI HHTG ERRY QCLA CGKS FINY QFMS SHIK SVHS QDPS GDSK LYRL HPCR SLQI RQYA YLSD RSST IPAM KDDG IGYK VDTG KEPP VGTT TSTQ NKPM TWED IFIQ QEND SIFK QNVT DGST EFEF IIPE SY
Tested Applications:WB ChIP ELISA
Recommended Dilution:
WB1:1000 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP5μg antibody for 10μg-15μg of Chromatin
Synonyms:ZNF348, ZNF-kaiso, Kaiso/ZBTB33
Positive Sample:U-87MG, Mouse kidney, Mouse skeletal muscle
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:74kDa
Observed MW:74-90kDa

This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Purification MethodAffinity purification
Gene ID10009
RRIDAB_2759745
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Kaiso/ZBTB33 Rabbit pAb (CAB12900) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageChromatin immunoprecipitation analysis of extracts of HeLa cells, using Kaiso/ZBTB33 antibody (CAB12900) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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