Epigenetics & Nuclear Signaling Antibodies 1

Prohibitin Rabbit Polyclonal Antibody (CAB0056)

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SKU:
CAB0056
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
PHB, HEL-215, HEL-S-54e, Prohibitin
Research Use:
Epigenetics and Nuclear Signaling
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Description

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Prohibitin Antibody (CAB0056)

The Prohibitin Antibody (CAB0056) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been validated for use in Western blot applications and is highly specific for detecting prohibitin in human samples.Prohibitin is known to be involved in the maintenance of mitochondrial function and has been implicated in diseases such as cancer and neurodegenerative disorders.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Prohibitin Antibody
SKU:CAB0056
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:PRNV PVIT GSKD LQNV NITL RILF RPVA SQLP RIFT SIGE DYDE RVLP SITT EILK SVVA RFDA GELI TQRE LVSR QVSD DLTE RAAT FGLI LDDV SLTH LTFG KEFT EAVE AKQV AQQE AERA RFVV EKAE QQKK AAII SAEG DSKA AELI ANSL ATAG DGLI ELRK LEAA EDIA YQLS RSRN ITYL PAGQ SVLL QLPQ
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PHB, HEL-215, HEL-S-54e, Prohibitin
Positive Sample:MCF7, 293T, Neuro-2a, Mouse liver, PC-12, Rat liver
Cellular Localization:Mitochondrion Inner Membrane.
Calculated MW:30kDa
Observed MW:30kDa

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID5245
RRIDAB_2756921
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Prohibitin Rabbit pAb (CAB0056) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Prohibitin Rabbit pAb (CAB0056) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using Prohibitin Rabbit pAb (CAB0056) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using Prohibitin Rabbit pAb (CAB0056) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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