Kaiso/ZBTB33 Rabbit Monoclonal Antibody (CAB3770)

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SKU:
CAB3770
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Rat
Synonyms:
ZNF348, ZNF-kaiso, Kaiso/ZBTB33
Research Use:
Epigenetics and Nuclear Signaling
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Description

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Kaiso/ZBTB33 Monoclonal Antibody (CAB3770)

The Kaiso/ZBTB33 Monoclonal Antibody (CAB3770) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting and immunohistochemistry.Kaiso, also known as ZBTB33, is a transcription factor involved in regulating gene expression and is implicated in various cellular processes, including cell proliferation, differentiation, and apoptosis.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:Kaiso/ZBTB33 Monoclonal Antibody
SKU:CAB3770
Size:20μL, 100μL
Reactivity:Human, Rat
Clone Number:ARC2086
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:HSQD PSGD SKLY RLHP CRSL QIRQ YAYL SDRS STIP AMKD DGIG YKVD TGKE PPVG TTTS TQNK PMTW EDIF IQQE NDSI FKQN VTDG STEF EFII PESY
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ZNF348, ZNF-kaiso, Kaiso/ZBTB33
Positive Sample:A-549, SH-SY5Y, Rat brain
Cellular Localization:Cytosol, Nucleolus, Nucleoplasm, Nucleus, Plasma Membrane.
Calculated MW:74KDa
Observed MW:80kDa

This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Purification MethodAffinity purification
Gene ID10009
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Kaiso/ZBTB33 Rabbit mAb (CAB3770) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of HeLa cells using Kaiso/ZBTB33 Rabbit mAb (CAB3770) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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