The CD3H Rabbit Monoclonal Antibody (CAB22473) from Assay Genie is a high-quality reagent designed for reliable detection of CD3H protein in human, mouse, and rat samples. CD3H, a component of the T-cell receptor (TCR) complex, plays a crucial role in T-cell activation and immune signaling. This antibody is validated for applications including Western blot (WB), immunohistochemistry on paraffin-embedded tissues (IHC-P), and ELISA. The kit optionally includes Bradford Reagent to enable accurate protein quantification and sample normalization. Manufactured and quality-controlled by Assay Genie, this product supports precise and reproducible immunological research and protein analysis.
Product Name:
CD3H Rabbit Monoclonal Antibody
SKU:
CAB22473
Size:
100μL
Species:
Rabbit
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC57083
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IHC-P
1:2000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene
Purification Method
Affinity purification
Gene ID
919
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. Store Bradford Reagent at Room Temperature for 1 Year.
Western blot analysis of various lysates, using CD3H Rabbit Monoclonal Antibody (CAB22473) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): K-562. Exposure time: 90s.
Western blot analysis of various lysates, using CD3H Rabbit Monoclonal Antibody (CAB22473) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD3H Rabbit Monoclonal Antibody (CAB22473) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
