Immunology Antibodies 2

CD3H Rabbit Polyclonal Antibody (CAB2058)

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SKU:
CAB2058
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
T3Z, CD3H, CD3Q, CD3Z, TCRZ, IMD25, CD3ZETA, CD3-ZETA
Research Use:
Immunology
Frequently bought together:

Description

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CD3H Antibody (CAB2058)

The CD3H Antibody (CAB2058) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CD247 protein, this antibody enables precise detection and analysis in various cell types, making it an invaluable resource for studies in immunology and cancer research.CD247, also known as the CD3 zeta chain, is a critical component of the T cell receptor complex and is essential for signal transduction during T cell activation.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CD3H Antibody
SKU:CAB2058
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:FLRV KFSR SADA PAYQ QGQN QLYN ELNL GRRE EYDV LDKR RGRD PEMG GKPQ RRKN PQEG LYNE LQKD KMAE AYSE IGMK GERR RGKG HDGL YQGL STAT KDTY DALH MQAL PPR
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:5000
IF/ICC1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:T3Z, CD3H, CD3Q, CD3Z, TCRZ, IMD25, CD3ZETA, CD3-ZETA
Positive Sample:Jurkat, Mouse thymus
Cellular Localization:Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:19kDa
Observed MW:19kDa

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID919
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CD3H Rabbit pAb (CAB2058) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophageal cancer using CD3H Rabbit pAb (CAB2058) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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