The CDC23 Monoclonal Antibody (CAB6025) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is optimized for use in Western blot applications. By targeting the CDC23 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies related to cell division and cancer research.CDC23, also known as cell division cycle protein 23, is essential for proper progression through the cell cycle and is involved in the regulation of cell growth and division.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDC23 Monoclonal Antibody
SKU:
CAB6025
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2133
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APC8, CUT23, ANAPC8, CDC23
Positive Sample:
HeLa, SKOV3, Mouse lung, Mouse brain, Mouse spleen, Rat brain
Cellular Localization:
Cytoplasm, Cytosol, Nucleoplasm.
Calculated MW:
69kDa
Observed MW:
69kDa
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.
Purification Method
Affinity purification
Gene ID
8697
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDC23 Rabbit mAb (CAB6025) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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