The RBX1 Antibody (CAB14500) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the RBX1 protein, this antibody enables precise detection and analysis in various cell types, making it an essential resource for studies in cell biology, cancer research, and the ubiquitin-proteasome pathway.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
RBX1 Antibody
SKU:
CAB14500
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ROC1, RNF75, BA554C12.1, RBX1
Positive Sample:
Mouse testis, Rat brain, Rat testis
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
12kDa
Observed MW:
12kDa
This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.
Purification Method
Affinity purification
Gene ID
9978
RRID
AB_2761374
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using RBX1 Rabbit pAb (CAB14500) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
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