Cell Biology Antibodies 1

VCAM1 Rabbit Polyclonal Antibody (CAB0279)

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SKU:
CAB0279
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
CD106, INCAM-100, VCAM1
Research Use:
Cell Biology
Frequently bought together:

Description

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VCAM1 Antibody (CAB0279)

The VCAM1 Antibody (CAB0279) is a high-quality antibody developed for reliable detection and analysis of target proteins. Produced in rabbits, this antibody exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the VCAM1 protein, this antibody facilitates the detection and analysis of VCAM1 expression in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.VCAM1, also known as vascular cell adhesion molecule 1, plays a crucial role in leukocyte adhesion and recruitment during inflammation.

This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:VCAM1 Antibody
SKU:CAB0279
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:NRKE VELI VQEK PFTV EISP GPRI AAQI GDSV MLTC SVMG CESP SFSW RTQI DSPL SGKV RSEG TNST LTLS PVSF ENEH SYLC TVTC GHKK LEKG IQVE L
Tested Applications:WB ELISA IF-P
Recommended Dilution:
WB1:500 - 1:1000
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CD106, INCAM-100, VCAM1
Positive Sample:HUVEC treated with hTNF-α, Mouse liver
Cellular Localization:Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:81kDa
Observed MW:95-120kDa

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.

Purification MethodAffinity purification
Gene ID7412
RRIDAB_2757091
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HUVEC cells, using VCAM1 Rabbit pAb (CAB0279) at 1:500 dilution. HUVEC cells were treated with Human Tumor Necrosis Factor-α (hTNF-α) (10 ng/ml) for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from Mouse liver, using VCAM1 Rabbit pAb (CAB0279) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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