Epigenetics & Nuclear Signaling Antibodies 4

PRP19 Rabbit Polyclonal Antibody (CAB6706)

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SKU:
CAB6706
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
PSO4, SNEV, PRP19, UBOX4, hPSO4, NMP200
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

PRP19 Antibody (CAB6706)

The PRP19 Antibody (CAB6706) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunofluorescence. By specifically binding to the PRPF19 protein, this antibody allows for the detection and analysis of PRPF19 in different cell types, making it a powerful tool for studies in molecular biology and gene expression.PRPF19 is essential for the accurate splicing of precursor messenger RNA (pre-mRNA) into mature mRNA, a crucial step in gene expression regulation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PRP19 Antibody
SKU:CAB6706
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSLI CSIS NEVP EHPC VSPV SNHV YERR LIEK YIAE NGTD PINN QPLS EEQL IDIK VAHP IRPK PPSA TSIP AILK ALQD EWDA VMLH SFTL RQQL QTTR QELS HALY QHDA ACRV IARL TKEV TAAR EALA TLKP QAGL IVPQ AVPS SQPS VVGA GEPM DLGE LVGM TPEI IQKL QDKA TVLT TERK KRGK TVPE ELVK PEEL SKYR QVAS HVGL HSAS
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PSO4, SNEV, PRP19, UBOX4, hPSO4, NMP200
Positive Sample:SW620
Cellular Localization:Cytoplasm, Lipid Droplet, Nucleus, Cytoskeleton, Nucleoplasm, Spindle.
Calculated MW:55kDa
Observed MW:60kDa

Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm; nuclear speck; and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.

Purification MethodAffinity purification
Gene ID27339
RRIDAB_2767289
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from SW620 cells, using PRP19 Rabbit pAb (CAB6706) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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