Epigenetics & Nuclear Signaling Antibodies 1

PRP19 Rabbit Polyclonal Antibody (CAB12590)

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SKU:
CAB12590
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
PSO4, SNEV, PRP19, UBOX4, hPSO4, NMP200
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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PRP19 Antibody (CAB12590)

The PRP19 Antibody (CAB12590) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the PRPF19 protein, this antibody enables precise detection and analysis of PRPF19 in a variety of cell types, making it ideal for studies in molecular biology and genetic research.PRPF19 plays a crucial role in the spliceosome complex, which is responsible for removing introns from pre-mRNA to produce functional mRNA.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PRP19 Antibody
SKU:CAB12590
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:AREA LATL KPQA GLIV PQAV PSSQ PSVV GAGE PMDL GELV GMTP EIIQ KLQD KATV LTTE RKKR GKTV PEEL VKPE ELSK YRQV ASHV GLHS ASIP GILA LDLC PSDT NKIL TGGA DKNV VVFD KSSE QILA TLKG HTKK VTSV VFHP SQDL VFSA SPDA TIRI WSVP NASC VQVV RAHE SAVT GLSL HATG DYLL SSSD DQYW AFSD IQTG RVLT KVTD ETSG CSLT CAQF HPDG LIFG TGTM DSQI KIWD LKER TNVA NFPG HSGP ITSI AFSE NGYY LATA ADDS SV
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PSO4, SNEV, PRP19, UBOX4, hPSO4, NMP200
Positive Sample:HepG2, Jurkat, HeLa, Mouse heart, Rat brain
Cellular Localization:Cytoplasm, Lipid Droplet, Nucleus, Cytoskeleton, Nucleoplasm, Spindle.
Calculated MW:55kDa
Observed MW:55kDa

Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm; nuclear speck; and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.

Purification MethodAffinity purification
Gene ID27339
RRIDAB_2759432
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PRP19 Rabbit pAb (CAB12590) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver using PRP19 Rabbit pAb (CAB12590) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human stomach using PRP19 Rabbit pAb (CAB12590) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using PRP19 Rabbit pAb (CAB12590) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of L929 cells using PRP19 Rabbit pAb (CAB12590) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using PRP19 Rabbit pAb (CAB12590) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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