The PRIM2 Antibody (CAB11633) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through rabbit immunization, exhibits high specificity and reactivity towards human samples, making it a reliable option for a variety of experimental applications. Validated for use in Western blotting, the PRIM2 antibody enables the precise detection and analysis of PRIM2 protein levels in different cell types, facilitating investigations in molecular biology and cancer research.PRIM2, also referred to as DNA primase subunit 2, plays a crucial role in the initiation of DNA synthesis by synthesizing short RNA primers that are essential for DNA polymerase to elongate DNA strands during replication.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PRIM2 Antibody
SKU:
CAB11633
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p58, PRIM2A, PRIM2
Positive Sample:
A375, H460, HeLa, HT-29, Mouse spleen
Cellular Localization:
Nucleoplasm.
Calculated MW:
59kDa
Observed MW:
59kDa
This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6.
Purification Method
Affinity purification
Gene ID
5558
RRID
AB_2758652
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRIM2 Rabbit pAb (CAB11633) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from mouse spleen, using PRIM2 Rabbit pAb (CAB11633) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
