Cell Biology Antibodies 2

ERp57 Rabbit Polyclonal Antibody (CAB1085)

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SKU:
CAB1085
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, PI-PLC, HsT17083, HEL-S-269, HEL-S-93n
Research Use:
Cell Biology
Frequently bought together:

Description

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ERp57 Antibody (CAB1085)

The ERp57 Antibody (CAB1085) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human PDIA3 and has been validated for use in Western blot applications. By binding to PDIA3, the antibody allows for detection and analysis of this important protein in a variety of cell types.PDIA3, also known as protein disulfide-isomerase A3, plays a crucial role in ensuring proper protein folding and assembly, as well as in regulating cellular redox balance.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ERp57 Antibody
SKU:CAB1085
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:FRPS HLTN KFED KTVA YTEQ KMTS GKIK KFIQ ENIF GICP HMTE DNKD LIQG KDLL IAYY DVDY EKNA KGSN YWRN RVMM VAKK FLDA GHKL NFAV ASRK TFSH ELSD FGLE STAG EIPV VAIR TAKG EKFV MQEE FSRD GKAL ERFL QDYF DGNL KRYL KSEP IPES NDGP VKVV VAEN FDEI VNNE NKDV LIEF YAPW CGHC KNLE PKYK ELGE KLSK DPNI VIAK MDAT ANDV PSPY EVRG FPTI YFSP ANKK LNPK KYEG GREL SDFI SYLQ REAT NPPV IQEE KPKK KKKA QEDL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, PI-PLC, HsT17083, HEL-S-269, HEL-S-93n
Positive Sample:NIH/3T3, MCF7, Jurkat, HepG2, LO2, Mouse brain, Mouse thymus, Mouse liver, Rat liver
Cellular Localization:Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome.
Calculated MW:54kDa
Observed MW:57kDa

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates.

Purification MethodAffinity purification
Gene ID2923
RRIDAB_2758270
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ERp57 Rabbit pAb (CAB1085) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human stomach using ERp57 Rabbit pAb (CAB1085) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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