Anti-ERp57 Antibody [Map.ERp57]

(No reviews yet) Write a Review
SKU:
STMA00042
Antibody Type:
Monoclonal
Applications:
WB
Applications:
IHC
Applications:
ICC/IF
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Bovine
Reactivity:
Dog
Reactivity:
Guinea Pig (Cavia porcellus)
Reactivity:
Hamster
Reactivity:
Monkey
Reactivity:
Pig
Reactivity:
Rabbit
Host Species:
Mouse
Isotype:
IgG1
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 (Grp58) Monoclonal Antibody, Clone Map.ERP57 (STMA00042). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Erp57 (Grp58) Monoclonal Antibody (STMA00042) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT.
  • Immunohistochemistry analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (STMA00042). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (STMA00042) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis and Hair Follicles.
  • Western Blot analysis of Human cell lysates showing detection of Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (STMA00042). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (STMA00042) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (STMA00042). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (STMA00042) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining.
Frequently bought together:

Description

ERp57 Antibody [Map.ERp57]

ERp57 Antibody [Map.ERp57] is a mouse monoclonal antibody directed against ERp57, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF and IP. Reported reactivity: Human, Mouse, Rat, Bovine, Dog, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig and Rabbit. Supplied as IgG1, purified by Protein G.

ERp57, also known as PDIA3, is a thiol-disulfide isomerase involved in protein folding and quality control within the endoplasmic reticulum (ER). In the brain, ERp57 plays a critical role in maintaining proteostasis, particularly under conditions of ER stress—a key feature of neurodegenerative diseases. ERp57 is upregulated in response to misfolded protein accumulation, as seen in Alzheimer’s, ALS, and prion diseases. It interacts with calnexin and calreticulin to assist in the proper folding of glycoproteins, including those involved in synaptic function and neuronal survival. Dysregulation of ERp57 impairs the unfolded protein response (UPR), contributing to neuronal dysfunction and cell death. Given its role in mitigating proteotoxic stress, ERp57 is emerging as a potential therapeutic target for enhancing cellular resilience in neurodegeneration. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 200 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:ERp57 Antibody [Map.ERp57]
Product SKU:STMA00042
Size:200 µg
Target:ERp57
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:Map.ERp57
Research Areas:Cell Signaling, Protein Trafficking, ER Proteins
Buffer:PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, IHC, ICC/IF, IP
Antibody Isotype:IgG1
Purification:Protein G Purified
Concentration:1 mg/ml
Reactivity:Human, Mouse, Rat, Bovine, Dog, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit
Recommended Dilution:WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~57kDa.
Guarantee:12 months from date of dispatch
Immunogen:Human recombinant ERp57 (Grp58)
Immunogen Species:Human
Accession Number:NP_005304.3
Gene ID:2923
Swiss-Prot:P30101
Cellular Localization:Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome
Alternative Names:ERp60, ERp61, Grp57, Grp58, P58, PDIA3, PI PLC, 58 kDa glucose regulated protein, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER 60, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, ER p57, ER protein 57, ER protein 60, ERp 57, ERp57, Glucose Regulated Protein 58 Kd, GRP 57, GRP 58, GRP57, HsT17083, p58, PDIA 3, PDIA3_HUMAN, Phospholipase C alpha, Protein disulfide isomerase A3, Protein disulfide isomerase family A member 3, Protein disulfide-isomerase A3
References:1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
Note:For research use only.
View AllClose

0 Reviews

View AllClose

Related Products

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 (STMA00076). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody (STMA00076) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9 (STMA00076). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (STMA00076) at 1:1000 for 2 hours at RT.

Anti-ERp57 Antibody [4F9]

ERp57 Antibody [4F9]ERp57 Antibody [4F9] is a mouse monoclonal antibody directed against ERp57, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported...