Cell Biology Antibodies 6

NGLY1 Rabbit Polyclonal Antibody (CAB15864)

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SKU:
CAB15864
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
CDDG, PNG1, CDG1V, PNG-1, PNGase, NGLY1
Research Use:
Cell Biology
Frequently bought together:

Description

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NGLY1 Antibody (CAB15864)

The NGLY1 Antibody (CAB15864) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds to the NGLY1 protein, enabling precise detection and analysis in a variety of cell types.NGLY1 deficiency is a complex disease that affects multiple systems in the body, including the nervous system and liver.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NGLY1 Antibody
SKU:CAB15864
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAAA ALGS SSGS ASPA VAEL CQNT PETF LEAS KLLL TYAD NILR NPND EKYR SIRI GNTA FSTR LLPV RGAV ECLF EMGF EEGE THLI FPKK ASVE QLQK IRDL IAIE RSSR LDGS NKSH KVKS SQQP AAST QLPT TPSS NPSG LNQH TRNR QGQS SDPP SAST VAAD SAIL EVLQ SNIQ HVLV YENP ALQE KALA CIPV QELK RKSQ EKLS RARK LDKG INIS DEDF LLLE LLHW FKEE FFHW VNNV LCSK CGGQ TRSR DRSL LPSD DELK WGAK EVED HYCD ACQF SNRF PRYN NPEK
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:5000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CDDG, PNG1, CDG1V, PNG-1, PNGase, NGLY1
Positive Sample:U266, K-562 , Mouse brain, Rat brain
Cellular Localization:Cytoplasm.
Calculated MW:74kDa
Observed MW:74kDa

This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID55768
RRIDAB_2763292
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NGLY1 Rabbit pAb (CAB15864) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunofluorescence analysis of PC-12 cells using NGLY1 Rabbit pAb (CAB15864) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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