Cell Death Antibodies 1

NFKB1 Rabbit Polyclonal Antibody (CAB14754)

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SKU:
CAB14754
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta, NFKB1
Research Use:
Cell Death
Frequently bought together:

Description

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NFKB1 Antibody (CAB14754)

The NFKB1 Antibody (CAB14754) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets NFkB1 and has been validated for use in Western blot applications. NFkB1 is a crucial regulator of gene expression in response to various stimuli, including pro-inflammatory cytokines and pathogens. Dysregulation of NFkB1 signaling has been implicated in a wide range of diseases, including cancer, autoimmune disorders, and inflammatory conditions.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NFKB1 Antibody
SKU:CAB14754
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GPYL QILE QPKQ RGFR FRYV CEGP SHGG LPGA SSEK NKKS YPQV KICN YVGP AKVI VQLV TNGK NIHL HAHS LVGK HCED GICT VTAG PKDM VVGF ANLG ILHV TKKK VFET LEAR MTEA CIRG YNPG LLVH PDLA YLQA EGGG DRQL GDRE KELI RQAA LQQT KEMD LSVV RLMF TAFL PDST GSFT RRLE PVVS DAIY DSKA PNAS NLKI VRMD RTAG CVTG GEEI YLLC DKVQ KDDI QIRF YEEE ENGG VWEG FGDF SPTD VHRQ FAIV FKTP KYKD INIT KPAS VFVQ LRRK SDLE TSEP KPFL YYPE IKDK EEVQ RKRQ K
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta, NFKB1
Positive Sample:Rat testis
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:105kDa
Observed MW:55kDa

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Purification MethodAffinity purification
Gene ID4790
RRIDAB_2761630
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Rat testis, using NFKB1 Rabbit pAb (CAB14754) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of BALB-3T3 cells using NFKB1 Rabbit pAb (CAB14754) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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