Cell Death Antibodies 1

NFKB1 Rabbit Polyclonal Antibody (CAB11160)

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SKU:
CAB11160
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Synonyms:
KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta, NFKB1
Research Use:
Cell Death
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Description

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NFKB1 Antibody (CAB11160)

The NFKB1 Antibody (CAB11160) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human NFkB1 and has been validated for use in Western blot applications.NFkB1 is a crucial regulator of immune and inflammatory responses, making it a target of interest in studies related to cancer, autoimmune diseases, and infectious diseases.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:NFKB1 Antibody
SKU:CAB11160
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:AAGA DPLV ENFE PLYD LDDS WENA GEDE GVVP GTTP LDMA TSWQ VFDI LNGK PYEP EFTS DDLL AQGD MKQL AEDV KLQL YKLL EIPD PDKN WATL AQKL GLGI LNNA FRLS PAPS KTLM DNYE VSGG TVRE LVEA LRQM GYTE AIEV IQAA SSPV KTTS QAHS LPLS PAST RQQI DELR DSDS VCDS GVET SFRK LSFT ESLT SGAS LLTL NKMP HDYG QEGP L
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:100 - 1:500
IHC-P1:50 - 1:200
IF/ICC1:20 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta, NFKB1
Positive Sample:Raji
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:105kDa
Observed MW:120kDa

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Purification MethodAffinity purification
Gene ID4790
RRIDAB_2758438
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Raji cells, using NFKB1 Rabbit pAb (CAB11160) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil using NFKB1 Rabbit pAb (CAB11160) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 150 μg extracts of MCF-7 cells using 3 μg NFKB1 antibody (CAB11160). Western blot was performed from the immunoprecipitate using NFKB1 antibody (CAB11160) at a dilution of 1:500.
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