KO Validated Antibodies 1

[KO Validated] NFKB1 Rabbit Polyclonal Antibody (CAB6667)

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SKU:
CAB6667
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta, NFKB1
Research Use:
Cell Death
Frequently bought together:

Description

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[KO Validated] NFKB1 Antibody (CAB6667)

The [KO Validated] NFKB1 Antibody (CAB6667) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is validated for use in various applications, including Western blotting, immunofluorescence, and immunohistochemistry.NFKB1, also known as NF-kappa-B p105, plays a crucial role in the activation of genes involved in immune and inflammatory signaling pathways. Dysregulation of NFKB1 has been implicated in a variety of diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] NFKB1 Antibody
SKU:CAB6667
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:GPYL QILE QPKQ RGFR FRYV CEGP SHGG LPGA SSEK NKKS YPQV KICN YVGP AKVI VQLV TNGK NIHL HAHS LVGK HCED GICT VTAG PKDM VVGF ANLG ILHV TKKK VFET LEAR MTEA CIRG YNPG LLVH PDLA YLQA EGGG DRQL GDRE KELI RQAA LQQT KEMD LSVV RLMF TAFL PDST GSFT RRLE PVVS DAIY DSKA PNAS NLKI VRMD RTAG CVTG GEEI YLLC DKVQ KDDI QIRF YEEE ENGG VWEG FGDF SPTD VHRQ FAIV FKTP KYKD INIT KPAS VFVQ LRRK SDLE TSEP KPFL YYPE IKDK EEVQ RKRQ K
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta, NFKB1
Positive Sample:Mouse lung
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:105kDa
Observed MW:50kDa/120kDa

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Purification MethodAffinity purification
Gene ID4790
RRIDAB_2863533
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse lung, using NFKB1 Rabbit pAb (CAB6667) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NFKB1 Rabbit pAb (CAB6667) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using NFKB1 Rabbit pAb (CAB6667) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (CAB6667) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (CAB6667) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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