KO Validated Antibodies 1

[KO Validated] HNRNPA2B1 Rabbit Polyclonal Antibody (CAB1162)

(No reviews yet) Write a Review
SKU:
CAB1162
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, IBMPFD2, HNRPA2B1, B1
Research Use:
Immunology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

[KO Validated] HNRNPA2B1 Antibody (CAB1162)

The [KO Validated] HNRNPA2B1 Antibody (CAB1162) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blot applications.HNRNPA2B1, also known as heterogeneous nuclear ribonucleoprotein A2/B1, is a key player in RNA metabolism and has been implicated in diseases such as cancer and neurodegenerative disorders. By targeting HNRNPA2B1, researchers can investigate its role in gene expression regulation, RNA splicing, and protein translation, providing insights into disease mechanisms and potential therapeutic targets.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] HNRNPA2B1 Antibody
SKU:CAB1162
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MEKT LETV PLER KKRE KEQF RKLF IGGL SFET TEES LRNY YEQW GKLT DCVV MRDP ASKR SRGF GFVT FSSM AEVD AAMA ARPH SIDG RVVE PKRA VARE ESGK PGAH VTVK KLFV GGIK EDTE EHHL RDYF EEYG KIDT IEII TDRQ SGKK RGFG FVTF DDHD PVDK IVLQ KYHT INGH
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IHC-P1:50 - 1:200
IF/ICC1:100 - 1:500
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, IBMPFD2, HNRPA2B1, B1
Positive Sample:A-549, HeLa, NIH/3T3, Rat spleen
Cellular Localization:Cytoplasm, Nucleus, Nucleoplasm.
Calculated MW:37kDa
Observed MW:36-38kDa

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.

Purification MethodAffinity purification
Gene ID3181
RRIDAB_2861609
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and HNRNPA2B1 knockout (KO) 293T cells, using [KO Validated] HNRNPA2B1 Rabbit pAb (CAB1162) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates, using [KO Validated] HNRNPA2B1 Rabbit pAb (CAB1162) at 1:3500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human cervix tissue using [KO Validated] HNRNPA2B1 Rabbit pAb (CAB1162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human brain tissue using [KO Validated] HNRNPA2B1 Rabbit pAb (CAB1162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of HeLa cells using [KO Validated] HNRNPA2B1 Rabbit pAb (CAB1162) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using [KO Validated] HNRNPA2B1 Rabbit pAb (CAB1162) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products