Cell Cycle Antibodies 1

GMNN Rabbit Polyclonal Antibody (CAB5316)

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SKU:
CAB5316
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
Gem, MGORS6, GMNN
Research Use:
Cell Cycle
Frequently bought together:

Description

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GMNN Antibody (CAB5316)

The GMNN Antibody (CAB5316) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blotting applications. It specifically binds to the Geminin protein, allowing for accurate detection and analysis in various cell types.Geminin is known for its important role in preventing re-replication of DNA during the cell cycle, making it a critical protein in maintaining genomic stability.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:GMNN Antibody
SKU:CAB5316
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MNPS MKQK QEEI KENI KNSS VPRR TLKM IQPS ASGS LVGR ENEL SAGL SKRK HRND HLTS TTSS PGVI VPES SENK NLGG VTQE SFDL MIKE NPSS QYWK EVAE KRRK ALYE ALKE NEKL HKEI EQKD NEIA RLKK ENKE LAEV AEHV QYMA ELIE RLNG EPLD NFES LDNQ EFDS EEET VEDS LVED SEIG TCAE GTVS SSTD AKPC I
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Gem, MGORS6, GMNN
Positive Sample:HeLa
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:24kDa
Observed MW:30kDa

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.

Purification MethodAffinity purification
Gene ID51053
RRIDAB_2766128
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using GMNN Rabbit pAb (CAB5316) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat brain using GMNN Rabbit pAb (CAB5316) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis using GMNN Rabbit pAb (CAB5316) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of MCF7 cells using GMNN Rabbit pAb (CAB5316) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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