Cell Death Antibodies 1

DAPK3 Rabbit Polyclonal Antibody (CAB15047)

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SKU:
CAB15047
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
DLK, ZIP, ZIPK, DAPK3
Research Use:
Cell Death
Frequently bought together:

Description

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DAPK3 Antibody (CAB15047)

The DAPK3 Antibody (CAB15047) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By specifically binding to the DAPK3 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in immunology and cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DAPK3 Antibody
SKU:CAB15047
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSTF RQED VEDH YEMG EELG SGQF AIVR KCRQ KGTG KEYA AKFI KKRR LSSS RRGV SREE IERE VNIL REIR HPNI ITLH DIFE NKTD VVLI LELV SGGE
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DLK, ZIP, ZIPK, DAPK3
Positive Sample:mouse brain, mouse lung, rat brain
Cellular Localization:Cytoplasm, Cytoplasm, Nucleus.
Calculated MW:53kDa
Observed MW:55kDa

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.

Purification MethodAffinity purification
Gene ID1613
RRIDAB_2761928
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using DAPK3 Rabbit pAb (CAB15047) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using DAPK3 Rabbit pAb (CAB15047) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using DAPK3 Rabbit pAb (CAB15047) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using DAPK3 Rabbit pAb (CAB15047) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of U-2 OS cells using DAPK3 Rabbit pAb (CAB15047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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