Signal Transduction Antibodies 3

ATP6V1B1 Rabbit Polyclonal Antibody (CAB6876)

(No reviews yet) Write a Review
SKU:
CAB6876
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
VATB, VMA2, VPP3, DRTA2, RTA1B, ATP6B1, ATP6V1B1
Research Use:
Signal Transduction
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

ATP6V1B1 Antibody (CAB6876)

The ATP6V1B1 Antibody (CAB6876) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ATP6V1B1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology, cell biology, and biochemistry.

This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:ATP6V1B1 Antibody
SKU:CAB6876
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAME IDSR PGGL PGSS CNLG AARE HMQA VTRN YITH PRVT YRTV CSVN GPLV VLDR VKFA QYAE IVHF TLPD GTQR SGQV LEVA GTKA IVQV FEGT SGID ARKT TCEF TGDI LRTP VSED MLGR VFNG SGKP IDKG PVVM AEDF LDIN GQPI NPHS RIYP EEMI QTGI SPID VMNS IARG QKIP IFSA AGLP HNEI AAQI CRQA GLVK KSKA VLDY HDDN FAIV FAAM GVNM ETAR FFKS DFEQ NGTM GNVC LFLN LAND PTIE RIIT PRLA LTTA EFLA
Tested Applications:WB ELISA IF-P
Recommended Dilution:
WB1:500 - 1:2000
IF-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:VATB, VMA2, VPP3, DRTA2, RTA1B, ATP6B1, ATP6V1B1
Positive Sample:BT-474, MCF7, HepG2, Mouse testis, Mouse kidney
Cellular Localization:Endomembrane System, Peripheral Membrane Protein.
Calculated MW:57kDa
Observed MW:57kDa

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness.

Purification MethodAffinity purification
Gene ID525
RRIDAB_2767436
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using ATP6V1B1 Rabbit pAb (CAB6876) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunofluorescence analysis of paraffin-embedded rat kidney using ATP6V1B1 Rabbit pAb (CAB6876) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products