Kinases

Kinases

  • Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody (STPA00077). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody (STPA00077) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT.

    Anti-PIK3CA Antibody

    PIK3CA AntibodyPIK3CA Antibody is a rabbit polyclonal antibody directed against PIK3CA, offered by Assay Genie for research applications. Reported applications include WB. Reported reactivity: Mouse...
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK1 Polyclonal Antibody (STPA00063). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (STPA00063) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (STPA00063). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa (15ug). Lane 3: Human 293Trap (15ug). Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (STPA00063) at 1:1000 for 1 hour at RT.

    Anti-ULK1 Antibody

    ULK1 AntibodyULK1 Antibody is a rabbit polyclonal antibody directed against ULK1, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity:...
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (STPA00027). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (STPA00027) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Western blot analysis of Human cancer cell lines showing detection of p38 protein using Rabbit Anti-p38 Polyclonal Antibody (STPA00027). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (STPA00027) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

    Anti-p38 Antibody

    p38 Antibodyp38 Antibody is a rabbit polyclonal antibody directed against p38, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF and IP. Reported...
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal Antibody (STPA00016). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody (STPA00016) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (STPA00016). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (STPA00016) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

    Anti-Erk1/2 Antibody

    Erk1/2 AntibodyErk1/2 Antibody is a rabbit polyclonal antibody directed against Erk1/2, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF and FCM...
  • Western blot analysis with Rabbit Anti-CASK Monoclonal Antibody, Clone PT55 (STMA00230) showing detection of Human CASK protein in a transient over-expression (OE) lysate (OriGene LY401218). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Human CASK Recombinant Monoclonal [PT55] (STMA00230) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis Rabbit Anti-CASK Monoclonal Antibody, Clone PT55 (STMA00230). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Human CASK Recombinant Monoclonal [PT55] (STMA00230) at 1:100 for 1 hour at RT.

    Anti-CASK Antibody [PT55]

    CASK Antibody [PT55]CASK Antibody [PT55] is a rabbit recombinant monoclonal antibody directed against CASK, offered by Assay Genie for research applications. Reported applications include WB, IHC and...
  • Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (STMA00031). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (STMA00031) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute. Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (STMA00031). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (STMA00031) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.

    Anti-p38 alpha MAPK Antibody [9F12]

    p38 alpha MAPK Antibody [9F12]p38 alpha MAPK Antibody [9F12] is a mouse monoclonal antibody directed against p38 alpha MAPK, offered by Assay Genie for research applications. Reported applications...
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (STMA00016). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (STMA00016) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (STMA00016). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (STMA00016) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.

    Anti-CaMKII Antibody [22B1]

    CaMKII Antibody [22B1]CaMKII Antibody [22B1] is a mouse monoclonal antibody directed against CaMKII, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF,...
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (STMA00015). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (STMA00015) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining. Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (STMA00015). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (STMA00015) at 1:1000.

    Anti-CaMKII alpha Antibody [6G9]

    CaMKII alpha Antibody [6G9]CaMKII alpha Antibody [6G9] is a mouse monoclonal antibody directed against CaMKII alpha, offered by Assay Genie for research applications. Reported applications include...