Anti-p38 alpha MAPK Antibody [9F12]

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SKU:
STMA00031
Antibody Type:
Monoclonal
Applications:
WB
Applications:
IHC
Applications:
IP
Applications:
ELISA
Applications:
AM
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Mouse
Isotype:
IgG1
  • Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (STMA00031). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (STMA00031) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.
  • Immunohistochemistry analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (STMA00031). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (STMA00031) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
  • Western Blot analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 (STMA00031). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody (STMA00031) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
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Description

p38 alpha MAPK Antibody [9F12]

p38 alpha MAPK Antibody [9F12] is a mouse monoclonal antibody directed against p38 alpha MAPK, offered by Assay Genie for research applications. Reported applications include WB, IHC, IP, ELISA and AM. Reported reactivity: Human, Mouse and Rat. Supplied as IgG1, purified by Protein G.

p38 MAPK (also known as SAPK2α) is a stress-activated kinase that orchestrates cellular responses to inflammatory and oxidative stimuli. It is activated by phosphorylation in response to diverse stressors, including hydrogen peroxide, cytokines, UV light, and chemotherapeutic agents. In neurons, p38 MAPK regulates apoptosis, synaptic remodeling, and inflammatory gene expression. Its activation leads to mitochondrial cytochrome c release and caspase-3 activation, contributing to neurodegeneration. Elevated p38 MAPK activity has been observed in Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis. Given its central role in stress signaling and cell death, p38 MAPK is a validated target for therapeutic intervention in neurodegenerative and inflammatory CNS disorders. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:p38 alpha MAPK Antibody [9F12]
Product SKU:STMA00031
Size:100 µg
Target:p38 alpha MAPK
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:9F12
Research Areas:Cell Signaling, Post-translational Modifications, Phosphorylation, Cancer
Buffer:PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, IHC, IP, ELISA, AM
Antibody Isotype:IgG1
Purification:Protein G Purified
Concentration:1 mg/ml
Reactivity:Human, Mouse, Rat
Recommended Dilution:WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~38kDa.
Guarantee:12 months from date of dispatch
Immunogen:Full length recombinant protein expressed in E.coli cells
Immunogen Species:Human
Accession Number:NP_001306.1
Gene ID:1432
Swiss-Prot:Q16539
Cellular Localization:Cytoplasm | Nucleus
Alternative Names:CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, MAPK14, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A
References:1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.318(1): 99-107.
Note:For research use only.
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