Epigenetics & Nuclear Signaling Antibodies 2

WT1 Rabbit Polyclonal Antibody (CAB16298)

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SKU:
CAB16298
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
GUD, AWT1, WAGR, WT-1, WT33, NPHS4, WIT-2, WT1
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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WT1 Antibody (CAB16298)

The WT1 Antibody (CAB16298) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.WT1 is a transcription factor that is frequently overexpressed in various cancers, making it a potential target for cancer therapy.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:WT1 Antibody
SKU:CAB16298
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MEKG YSTV TFDG TPSY GHTP SHHA AQFP NHSF KHED PMGQ QGSL GEQQ YSVP PPVY GCHT PTDS CTGS QALL LRTP YSSD NLYQ MTSQ LECM TWNQ MNLG ATLK GVAA GSSS SVKW TEGQ SNHS TGYE SDNH TTPI LCGA QYRI HTHG VFRG IQDV RRVP GVAP TLVR SASE TSEK RPFM CAYP GCNK RYFK LSHL QMHS RKHT GEKP YQCD FKDC ERRF SRSD QLKR HQRR HTGV KPFQ CKTC QRKF SRSD HLKT HTRT HTGE KPFS CRWP SCQK KFAR SDEL VRHH NMHQ RNMT KLQL AL
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GUD, AWT1, WAGR, WT-1, WT33, NPHS4, WIT-2, WT1
Positive Sample:Mouse uterus, Rat kidney, Rat testis
Cellular Localization:Cytoplasm, Nucleus, Nucleus Speckle, Nucleolus, Nucleoplasm.
Calculated MW:49kDa
Observed MW:49-60kDa

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Purification MethodAffinity purification
Gene ID7490
RRIDAB_2772912
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using WT1 Rabbit pAb (CAB16298) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageConfocal immunofluorescence analysis of Hela cells using WT1 Rabbit pAb (CAB16298) at dilution of 1:200. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of OVCAR-3 cells using WT1 Rabbit pAb (CAB16298) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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