KO Validated Antibodies 1

[KO Validated] VAT1 Rabbit Polyclonal Antibody (CAB17077)

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SKU:
CAB17077
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
VATI, T1
Research Use:
Cell Biology
Frequently bought together:

Description

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[KO Validated] VAT1 Antibody (CAB17077)

The [KO Validated] VAT1 Antibody (CAB17077) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits, is highly specific to human samples and is approved for use in Western blot applications. By binding to Vat1, this antibody enables researchers to detect and analyze the protein in various cell types, making it ideal for studies in cell biology and neuroscience.Vat1 is a key player in the transport of vesicles within cells, playing a crucial role in maintaining cellular function and communication.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] VAT1 Antibody
SKU:CAB17077
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:GVGM AAVQ LCRT VENV TVFG TASA SKHE ALKE NGVT HPID YHTT DYVD EIKK ISPK GVDI VMDP LGGS DTAK GYNL LKPM GKVV TYGM ANLL TGPK RNLM A
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:VATI, T1
Positive Sample:K-562, U-87MG, 293T
Cellular Localization:Extracellular Exosome, Extracellular Region, Mitochondrial Outer Membrane.
Calculated MW:42kDa
Observed MW:42kDa

Synaptic vesicles are responsible for regulating the storage and release of neurotransmitters in the nerve terminal. The protein encoded by this gene is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins.

Purification MethodAffinity purification
Gene ID10493
RRIDAB_2772842
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] VAT1 Rabbit pAb (CAB17077) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5min.
Product ImageWestern blot analysis of lysates from wild type (WT) and VAT1 knockout (KO) 293T cells, using [KO Validated] VAT1 Rabbit pAb (CAB17077) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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