KO Validated Antibodies 1

[KO Validated] NDUFA5 Rabbit Polyclonal Antibody (CAB14751)

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SKU:
CAB14751
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
B13, NUFM, UQOR13, CI-13kB, CI-13KD-B, NDUFA5
Research Use:
Cell Biology
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Description

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[KO Validated] NDUFA5 Antibody (CAB14751)

The [KO Validated] NDUFA5 Antibody (CAB14751) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human NDUFA5 protein and is validated for use in Western blotting applications. By binding to NDUFA5, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in mitochondrial function and energy metabolism.NDUFA5 is essential for the proper function of complex I in the electron transport chain, playing a key role in ATP production and cellular respiration.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] NDUFA5 Antibody
SKU:CAB14751
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAGV LKKT TGLV GLAV CNTP HERL RILY TKIL DVLE EIPK NAAY RKYT EQIT NEKL AMVK AEPD VKKL EDQL QGGQ LEEV ILQA EHEL NLAR KMRE WKLW EPLV EEPP ADQW KWPI
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:100
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:B13, NUFM, UQOR13, CI-13kB, CI-13KD-B, NDUFA5
Positive Sample:293T, Jurkat, NCI-H460
Cellular Localization:Matrix Side, Mitochondrion Inner Membrane, Peripheral Membrane Protein.
Calculated MW:13kDa
Observed MW:13kDa

This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.

Purification MethodAffinity purification
Gene ID4698
RRIDAB_2861705
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NDUFA5 Rabbit pAb (CAB14751) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil using NDUFA5 Rabbit pAb (CAB14751) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human gastric cancer using NDUFA5 Rabbit pAb (CAB14751) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH-3T3 cells using NDUFA5 Rabbit pAb (CAB14751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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