KO Validated Antibodies 1

[KO Validated] EGFR Rabbit Polyclonal Antibody (CAB2069)

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SKU:
CAB2069
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2, EGFR
Research Use:
Immunology
Frequently bought together:

Description

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[KO Validated] EGFR Antibody (CAB2069)

The [KO Validated] EGFR Antibody (CAB2069) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the EGFR protein, enabling accurate detection and analysis in a variety of cell types, making it ideal for studies in cancer biology and signal transduction pathways.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] EGFR Antibody
SKU:CAB2069
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:RPAG SVQN PVYH NQPL NPAP SRDP HYQD PHST AVGN PEYL NTVQ PTCV NSTF DSPA HWAQ KGSH QISL DNPD YQQD FFPK EAKP NGIF KGST AENA EYLR V
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IP0.5μg-4μg antibody for 400μg-800μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2, EGFR
Positive Sample:HeLa, Mouse liver, Rat brain, Rat liver, Rat liver, Rat liver
Cellular Localization:Cell Membrane, Endoplasmic Reticulum Membrane, Endosome, Endosome Membrane, Golgi Apparatus Membrane, Nucleus Membrane, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:134kDa
Observed MW:175kDa//175kDa/175kDa

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Purification MethodAffinity purification
Gene ID1956
RRIDAB_2764092
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using EGFR Rabbit pAb (CAB2069) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of lysates from Rat brain, using EGFR Rabbit pAb (CAB2069) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Product ImageImmunoprecipitation of EGFR from 600 µg extracts of Rat liver tissue was performed using 3 µg of EGFR Rabbit pAb (CAB2069). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using EGFR Rabbit pAb (CAB2069) at a dilution of 1:1000.
Product ImageImmunoprecipitation of EGFR from 600 µg extracts of Rat liver tissue was performed using 3 µg of EGFR Rabbit pAb (CAB2069). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using EGFR Rabbit pAb (CAB2069) at a dilution of 1:1000.
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