KO Validated Antibodies 1

[KO Validated] EGFR Rabbit Polyclonal Antibody (CAB11351)

(No reviews yet) Write a Review
SKU:
CAB11351
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2, EGFR
Research Use:
Immunology
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

[KO Validated] EGFR Antibody (CAB11351)

The [KO Validated] EGFR Antibody (CAB11351) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been extensively validated for its specificity and sensitivity in various research applications, including Western blotting.EGFR is a transmembrane tyrosine kinase receptor that plays a crucial role in regulating cell growth and differentiation. Dysregulation of EGFR signaling is commonly observed in various cancers, making it an attractive target for cancer therapy. The KO-Validated EGFR Polyclonal Antibody specifically binds to EGFR, enabling researchers to detect and analyze its expression in different cell types and tissues.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] EGFR Antibody
SKU:CAB11351
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:QGFF SSPS TSRT PLLS SLSA TSNN STVA CIDR NGLQ SCPI KEDS FLQR YSSD PTGA LTED SIDD TFLP VPEY INQS VPKR PAGS VQNP VYHN QPLN PAPS RDPH YQDP HSTA VGNP EYLN TVQP TCVN STFD SPAH WAQK GSHQ ISLD NPDY QQDF FPKE AKPN GIFK GSTA ENAE YLRV APQS SEFI GA
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IHC-P1:50 - 1:200
IF/ICC1:100 - 1:500
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2, EGFR
Positive Sample:A431
Cellular Localization:Cell Membrane, Endoplasmic Reticulum Membrane, Endosome, Endosome Membrane, Golgi Apparatus Membrane, Nucleus Membrane, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:134kDa
Observed MW:175kDa

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Purification MethodAffinity purification
Gene ID1956
RRIDAB_2861549
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from A431 cells, using EGFR Rabbit pAb (CAB11351) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver using EGFR Rabbit pAb (CAB11351) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse heart using EGFR Rabbit pAb (CAB11351) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of A-431 cells using EGFR Rabbit pAb (CAB11351) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products