KO Validated Antibodies 1

[KO Validated] STAT3 Rabbit Polyclonal Antibody (CAB1192)

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SKU:
CAB1192
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse, Rat
Synonyms:
APRF, HIES, ADMIO, ADMIO1, STAT3
Research Use:
Immunology
Frequently bought together:

Description

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[KO Validated] STAT3 Antibody (CAB1192)

The [KO Validated] STAT3 Antibody (CAB1192) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the STAT3 protein, this antibody enables the detection and analysis of STAT3 in various cell types, making it ideal for investigations in fields such as cancer research and immunology.STAT3 is a transcription factor that plays a critical role in various cellular processes, including cell proliferation, differentiation, and apoptosis.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] STAT3 Antibody
SKU:CAB1192
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:TKPP GTFL LRFS ESSK EGGV TFTW VEKD ISGK TQIQ SVEP YTKQ QLNN MSFA EIIM GYKI MDAT NILV SPLV YLYP DIPK EEAF GKYC RPES QEHP EADP G
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:5000
IF/ICC1:50 - 1:200
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:APRF, HIES, ADMIO, ADMIO1, STAT3
Positive Sample:A-549, HeLa
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:88kDa
Observed MW:86kDa

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.

Purification MethodAffinity purification
Gene ID6774
RRIDAB_2861642
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Product ImageWestern blot analysis of various lysates, using STAT3 Rabbit pAb (CAB1192) at 1:2500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using STAT3 Rabbit pAb (CAB1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat heart using STAT3 Rabbit pAb (CAB1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using STAT3 Rabbit pAb (CAB1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (CAB1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (CAB1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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