KO Validated Antibodies 1

[KO Validated] KDM5B Rabbit Polyclonal Antibody (CAB7772)

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SKU:
CAB7772
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
CT31, PLU1, PUT1, MRT65, PLU-1, JARID1B, PPP1R98, RBP2-H1, RBBP2H1A, 5B
Research Use:
Cell Biology
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Description

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[KO Validated] KDM5B Antibody (CAB7772)

The [KO Validated] KDM5B Antibody (CAB7772) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high specificity and sensitivity in detecting KDM5B in human samples, and has been validated for use in Western blot applications.KDM5B, also known as JARID1B, is a member of the Jumonji family of histone demethylases and plays a critical role in regulating gene expression by removing methyl groups from histone proteins. Dysregulation of KDM5B has been linked to various diseases, including cancer, neurodevelopmental disorders, and metabolic syndromes, making it a promising target for therapeutic interventions.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] KDM5B Antibody
SKU:CAB7772
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:EESE MKKF PDND LLRH LRLV TQDA EKCA SVAQ QLLN GKRQ TRYR SGGG KSQN QLTV NELR QFVT QLYA LPCV LSQT PLLK DLLN RVED FQQH SQKL LSEE
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CT31, PLU1, PUT1, MRT65, PLU-1, JARID1B, PPP1R98, RBP2-H1, RBBP2H1A, 5B
Positive Sample:293T
Cellular Localization:Nucleus.
Calculated MW:176kDa
Observed MW:176kDa

This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID10765
RRIDAB_2770071
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and KDM5B knockout (KO) 293T cells, using [KO Validated] KDM5B Rabbit pAb (CAB7772) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunofluorescence analysis of L929 cells using [KO Validated] KDM5B Rabbit pAb (CAB7772) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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