KO Validated Antibodies 1

[KO Validated] NDUFS3 Rabbit Polyclonal Antibody (CAB8013)

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SKU:
CAB8013
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
CI-30, MC1DN8, NDUFS3
Research Use:
Cell Biology
Frequently bought together:

Description

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[KO Validated] NDUFS3 Antibody (CAB8013)

The [KO Validated] NDUFS3 Antibody (CAB8013) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. With its ability to bind specifically to the NDUFS3 protein, researchers can accurately detect and analyze this key component of mitochondrial function in various cell types.NDUFS3 is essential for the function of complex I in the electron transport chain, making it crucial for cellular energy production.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] NDUFS3 Antibody
SKU:CAB8013
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ESAG ADTR PTVR PRND VAHK QLSA FGEY VAEI LPKY VQQV QVSC FNEL EVCI HPDG VIPV LTFL RDHT NAQF KSLV DLTA VDVP TRQN RFEI VYNL LSLR FNSR IRVK TYTD ELTP IESA VSVF KAAN WYER EIWD MFGV FFAN HPDL RRIL TDYG FEGH PFRK DFPL SGYV ELRY DDEV KRVV AEPV ELAQ EFRK FDLN SPWE AFPV YRQP PESL KLEA GDKK PDAK
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CI-30, MC1DN8, NDUFS3
Positive Sample:HepG2, Mouse kidney, Mouse heart, Mouse skeletal muscle, Rat kidney, Rat heart, Rat skeletal muscle, MCF7, Rat heart
Cellular Localization:Mitochondrion Inner Membrane.
Calculated MW:30kDa
Observed MW:26kDa/30kDa

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Purification MethodAffinity purification
Gene ID4722
RRIDAB_2863565
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using NDUFS3 Rabbit pAb (CAB8013) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunoprecipitation analysis of 600 μg extracts of Mouse kidney using 3 μg NDUFS3 antibody (CAB8013). Western blot was performed from the immunoprecipitate using NDUFS3 antibody (CAB8013) at a dilution of 1:1000.
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