KO Validated Antibodies 1

[KO Validated] HADHA Rabbit Polyclonal Antibody (CAB5346)

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SKU:
CAB5346
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
GBP, ECHA, HADH, LCEH, MTPA, LCHAD, TP-ALPHA, HA
Research Use:
Metabolism
Frequently bought together:

Description

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[KO Validated] HADHA Antibody (CAB5346)

The [KO Validated] HADHA Antibody (CAB5346) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications.HADHA, also known as Trifunctional enzyme subunit alpha, is essential for the beta-oxidation of long-chain fatty acids and helps maintain energy balance in cells. Dysregulation of HADHA has been linked to metabolic disorders such as fatty acid oxidation disorders and mitochondrial diseases.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] HADHA Antibody
SKU:CAB5346
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:IAAV SKRP EKVI GMHY FSPV DKMQ LLEI ITTE KTSK DTSA SAVA VGLK QGKV IIVV KDGP GFYT TRCL APMM SEVI RILQ EGVD PKKL DSLT TSFG FPVG AATL VDEV GVDV AKHV AEDL GKVF GERF GGGN PELL TQMV SKGF LGRK SGKG FYIY QEGV KRKD LNSD MDSI LASL KLPP KSEV SSDE DIQF RLVT RFVN EAVM CLQE GILA TPAE GDIG AVFG LGFP PCLG GPFR FVDL YGAQ KIVD RLKK YEAA YGKQ FTPC QLLA DHAN SPNK KFYQ
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:2000 - 1:6000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GBP, ECHA, HADH, LCEH, MTPA, LCHAD, TP-ALPHA, HA
Positive Sample:293T, K-562
Cellular Localization:Mitochondrion.
Calculated MW:83kDa
Observed MW:78kDa

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

Purification MethodAffinity purification
Gene ID3030
RRIDAB_2863499
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from K-562 cells, using [KO Validated] HADHA Rabbit pAb (CAB5346) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of lysates from wild type (WT) and HADHA knockout (KO) 293T cells, using [KO Validated] HADHA Rabbit pAb (CAB5346) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of A-549 cells using [KO Validated] HADHA Rabbit pAb (CAB5346). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg HADHA antibody (CAB5346). Western blot was performed from the immunoprecipitate using HADHA antibody (CAB5346) at a dilution of 1:1000.
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