Metabolism Antibodies 1

SREBP1 Rabbit Polyclonal Antibody (CAB15586)

(No reviews yet) Write a Review
SKU:
CAB15586
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
HMD, IFAP2, SREBP1, bHLHd1, SREBF1
Research Use:
Metabolism
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

SREBP1 Antibody (CAB15586)

The SREBP1 Antibody (CAB15586) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting SREBF1 in human samples, making it ideal for use in Western blot applications.SREBF1, also known as sterol regulatory element binding transcription factor 1, plays a crucial role in the control of cholesterol and fatty acid synthesis in cells. Dysregulation of SREBF1 has been implicated in various metabolic disorders, including obesity, diabetes, and cardiovascular diseases.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:SREBP1 Antibody
SKU:CAB15586
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:This information is considered to be commercially sensitive.
Sequence:SLHT QVQS VVPQ QLLT VTAA PTAA PVTT TVTS QIQQ VPVL LQPH FIKA DSLL LTAM KTDG ATVK AAGL SPLV SGTT VQTG PLPT LVSG GTIL ATVP LVVD AEKL PINR LAA
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HMD, IFAP2, SREBP1, bHLHd1, SREBF1
Positive Sample:Rat liver
Cellular Localization:Copii-Coated Vesicle Membrane, Cytoplasmic Vesicle, Endoplasmic Reticulum Membrane, Golgi Apparatus Membrane, Multi-Pass Membrane Protein, Multi-Pass Membrane Protein, Nucleus.
Calculated MW:122kDa
Observed MW:68kDa

This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively.

Purification MethodAffinity purification
Gene ID6720
RRIDAB_2762991
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Rat liver using SREBF1 Rabbit pAb (CAB15586) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of C6 cells using SREBF1 Rabbit pAb (CAB15586) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products