Cell Cycle Antibodies 2

SPAG8 Rabbit Polyclonal Antibody (CAB8943)

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SKU:
CAB8943
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Antibody Type:
Polyclonal Antibody
Reactivity:
Mouse, Rat
Synonyms:
SMP1, BS-84, CT142, HSD-1, SPAG3, CILD28, hSMP-1, SPAG8
Research Use:
Cell Cycle
Frequently bought together:

Description

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SPAG8 Antibody (CAB8943)

The SPAG8 Antibody (CAB8943) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in various applications, including Western blotting. By binding to the SPAG8 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in reproductive biology and infertility research.SPAG8 is known for its role in sperm function and male fertility, making it a key target for investigations into reproductive health and infertility issues.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.

Product Name:SPAG8 Antibody
SKU:CAB8943
Size:20μL, 100μL
Reactivity:Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LVAD RVPN YTSW SQHC PWEP QKQP PWEF LQVL EPGA RGLW KPPD IKGK LMVC YETL PRGQ CLLY NWEE ERAT NHLD QVPS MQDG SESF FFRH GHRG LLTM QLKS PMPS STTQ KDSY QPPG NVYW PLRG KREA MLEM LLQH QICK EVQA EQEP TRKL FEVE SVTH HDYR MELA QAGT PAPT KPHD YRQE QPET FWIQ RAPQ L
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:SMP1, BS-84, CT142, HSD-1, SPAG3, CILD28, hSMP-1, SPAG8
Positive Sample:Mouse testis, Rat testis
Cellular Localization:Cytoplasmic Vesicle, Acrosome, Secretory Vesicle.
Calculated MW:51kDa
Observed MW:45kDa

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Purification MethodAffinity purification
Gene ID26206
RRIDAB_2772359
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using SPAG8 Rabbit pAb (CAB8943) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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