Developmental Biology

SFRP2 Rabbit Polyclonal Antibody (CAB5383)

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SKU:
CAB5383
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
Synonyms:
FRP-2, SARP1, SDF-5, SFRP2
Research Use:
Developmental Biology
Frequently bought together:

Description

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SFRP2 Antibody (CAB5383)

The SFRP2 Antibody (CAB5383) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the SFRP2 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential component in studies related to developmental biology, tissue regeneration, and cancer research.SFRP2, also known as secreted frizzled-related protein 2, is a key player in regulating Wnt signaling pathways, which are crucial for normal development and control of cell proliferation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.

Product Name:SFRP2 Antibody
SKU:CAB5383
Size:20μL, 100μL
Reactivity:Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LFLF GQPD FSYK RSNC KPIP ANLQ LCHG IEYQ NMRL PNLL GHET MKEV LEQA GAWI PLVM KQCH PDTK KFLC SLFA PVCL DDLD ETIQ PCHS LCVQ VKDR CAPV MSAF GFPW PDML ECDR FPQD NDLC IPLA SSDH LLPA TEEA PKVC EACK NKND DDND IMET LCKN DFAL KIKV KEIT YINR DTKI ILET KSKT IYKL NGVS ERDL KKSV LWLK DSLQ CTCE EMND INAP YLVM GQKQ GGEL VITS VKRW QKGQ REFK RISR SIRK LQC
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FRP-2, SARP1, SDF-5, SFRP2
Positive Sample:Mouse kidney, Mouse heart
Cellular Localization:Secreted.
Calculated MW:33kDa
Observed MW:35kDa

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Purification MethodAffinity purification
Gene ID6423
RRIDAB_2766193
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using SFRP2 Rabbit pAb (CAB5383) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody (STPA00085). Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody (STPA00085) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection. Western blot analysis of Mouse Brain showing detection of 33.5 kDa SFRP2 protein using Rabbit Anti-SFRP2 Polyclonal Antibody (STPA00085). Load: 15 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody (STPA00085) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT.

Anti-SFRP2 Antibody

SFRP2 AntibodySFRP2 Antibody is a rabbit polyclonal antibody directed against SFRP2, offered by Assay Genie for research applications. Reported applications include WB and IHC. Reported reactivity:...