Cell Cycle Antibodies 1

RUVBL1/TIP49A/PONTIN Rabbit Polyclonal Antibody (CAB5723)

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SKU:
CAB5723
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse
Synonyms:
RVB1, TIH1, ECP54, TIP49, ECP-54, INO80H, NMP238, PONTIN, TIP49A, NMP 238, Pontin52, RUVBL1/TIP49A/PONTIN
Research Use:
Cell Cycle
Frequently bought together:

Description

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RUVBL1/TIP49A/PONTIN Antibody (CAB5723)

The RUVBL1/TIP49A/PONTIN Antibody (CAB5723) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the RUVBL1 protein, this antibody allows for the detection and analysis of RUVBL1 in a variety of cell types, making it an excellent choice for studies in molecular biology and cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:RUVBL1/TIP49A/PONTIN Antibody
SKU:CAB5723
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MKIE EVKS TTKT QRIA SHSH VKGL GLDE SGLA KQAA SGLV GQEN AREA CGVI VELI KSKK MAGR AVLL AGPP GTGK TALA LAIA QELG SKVP FCPM VGSE VYST EIKK TEVL MENF RRAI GLRI KETK EVYE GEVT ELTP CETE NPMG GYGK TISH VIIG LKTA KGTK QLKL DPSI FESL QKER VEAG DVIY IEAN SGAV KRQG RCDT YATE FDLE AEEY VPLP KGDV HKKK EIIQ DVTL
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:20 - 1:50
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RVB1, TIH1, ECP54, TIP49, ECP-54, INO80H, NMP238, PONTIN, TIP49A, NMP 238, Pontin52, RUVBL1/TIP49A/PONTIN
Positive Sample:HL-60, MCF7, HepG2, 293T, Mouse spleen, Mouse skeletal muscle, Mouse thymus, Mouse brain
Cellular Localization:Cytoplasm, Membrane, Nucleus, Nucleus Matrix, Centrosome, Cytoskeleton, Microtubule Organizing Center, Nucleoplasm.
Calculated MW:50kDa
Observed MW:55kDa

This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID8607
RRIDAB_2766480
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RUVBL1/TIP49A/PONTIN Rabbit pAb (CAB5723) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 15s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human stomach using RUVBL1/TIP49A/PONTIN Rabbit pAb (CAB5723) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human leiomyoma of uterus using RUVBL1/TIP49A/PONTIN Rabbit pAb (CAB5723) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of A549 cells using RUVBL1/TIP49A/PONTIN Rabbit pAb (CAB5723). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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