Cell Cycle Antibodies 2

RUVBL1/TIP49A/PONTIN Rabbit Monoclonal Antibody (CAB5180)

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SKU:
CAB5180
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
RVB1, TIH1, ECP54, TIP49, ECP-54, INO80H, NMP238, PONTIN, TIP49A, NMP 238, Pontin52, RUVBL1/TIP49A/PONTIN
Research Use:
Cell Cycle
Frequently bought together:

Description

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RUVBL1/TIP49A/PONTIN Monoclonal Antibody (CAB5180)

The RUVBL1/TIP49A/PONTIN Monoclonal Antibody (CAB5180) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit monoclonal technology, exhibits high specificity and sensitivity in detecting TIP49A in various experimental settings. Validated for use in immunofluorescence and flow cytometry applications, it allows for precise visualization and quantification of TIP49A expression in different cell types.TIP49A, also known as RUVBL1, is a crucial component of chromatin remodeling complexes and is involved in diverse cellular processes such as transcriptional regulation, DNA repair, and cell cycle progression.

This antibody is validated for use in WB, IHC-P, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:RUVBL1/TIP49A/PONTIN Monoclonal Antibody
SKU:CAB5180
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC1247
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:IPLD LLDR VMII RTML YTPQ EMKQ IIKI RAQT EGIN ISEE ALNH LGEI GTKT TLRY SVQL LTPA NLLA KING KDSI EKEH VEEI SELF YDAK SSAK ILAD QQDK YMK
Tested Applications:WB IHC-P ChIP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
ChIP5μg antibody for 10μg-15μg of Chromatin
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RVB1, TIH1, ECP54, TIP49, ECP-54, INO80H, NMP238, PONTIN, TIP49A, NMP 238, Pontin52, RUVBL1/TIP49A/PONTIN
Positive Sample:U-2 OS, A-431, HT-1080, Mouse brain, Mouse spleen, Rat brain
Cellular Localization:Cytoplasm, Membrane, Nucleus, Nucleus Matrix, Centrosome, Cytoskeleton, Microtubule Organizing Center, Nucleoplasm.
Calculated MW:50kDa
Observed MW:51kDa

This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID8607
RRIDAB_2863477
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RUVBL1/TIP49A/PONTIN Rabbit mAb (CAB5180) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse testis using RUVBL1/TIP49A/PONTIN Rabbit mAb (CAB5180) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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