Epigenetics & Nuclear Signaling Antibodies 3

PRDM14 Rabbit Polyclonal Antibody (CAB5543)

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SKU:
CAB5543
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Applications:
IP
Applications:
ChIP
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
PFM11, PRDM14
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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PRDM14 Antibody (CAB5543)

The PRDM14 Antibody (CAB5543) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By binding to the PRDM14 protein, this antibody enables the detection and analysis of PRDM14 in various cell types, making it an ideal choice for studies in stem cell biology and cancer research.PRDM14 is a transcription factor that plays a crucial role in maintaining the pluripotency of embryonic stem cells and induced pluripotent stem cells.

This antibody is validated for use in WB, IHC-P, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PRDM14 Antibody
SKU:CAB5543
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MALP RPSE AVPQ DKVC YPPE SSPQ NLAA YYTP FPSY GHYR NSLA TVEE DFQP FRQL EAAA SAAP AMPP FPFR MAPP LLSP GLGL QREP LYDL PWYS KLPP WYPI PHVP REVP PFLS SSHE YAGA SSED LGHQ IIGG DNES GPCC GPDT LIPP PPAD ASLL PEGL RTSQ LLPC SPSK QSED GPKP SNQE GKSP ARFQ FTEE DLHF VLYG VTPS LEHP ASLH HAIS GLLV PPDS SGSD SLPQ TLDK DSLQ LPEG LCLM QTVF
Tested Applications:WB IHC-P ChIP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP5μg antibody for 10μg-15μg of Chromatin
Synonyms:PFM11, PRDM14
Positive Sample:NCI-H125, Mouse ovary, Rat lung
Cellular Localization:Nucleus.
Calculated MW:64kDa
Observed MW:70kDa

This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.

Purification MethodAffinity purification
Gene ID63978
RRIDAB_2766324
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PRDM14 Rabbit pAb (CAB5543) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageWestern blot analysis of lysates from NCI-H125 cells, using PRDM14 Rabbit pAb (CAB5543) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageChromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody (CAB5543) and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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