Epigenetics & Nuclear Signaling Antibodies 1

POLR2H Rabbit Polyclonal Antibody (CAB13339)

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SKU:
CAB13339
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
RPB8, RPB17, RPABC3, POLR2H
Research Use:
Epigenetics and Nuclear Signaling
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Description

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POLR2H Antibody (CAB13339)

The POLR2H Antibody (CAB13339) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POLR2H protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and gene expression research.POLR2H is essential for the transcription of protein-coding genes and is involved in various cellular processes, making it a target of interest in studies related to gene regulation, RNA synthesis, and transcriptional control.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:POLR2H Antibody
SKU:CAB13339
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MAGI LFED IFDV KDID PEGK KFDR VSRL HCES ESFK MDLI LDVN IQIY PVDL GDKF RLVI ASTL YEDG TLDD GEYN PTDD RPSR ADQF EYVM YGKV YRIE GDET STEA ATRL SAYV SYGG LLMR LQGD ANNL HGFE VDSR VYLL MKKL AF
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RPB8, RPB17, RPABC3, POLR2H
Positive Sample:SW480, MCF7, HL-60, 293T, Mouse spleen, Mouse thymus
Cellular Localization:Nucleus, Nucleolus.
Calculated MW:17kDa
Observed MW:17kDa

The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.

Purification MethodAffinity purification
Gene ID5437
RRIDAB_2760195
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using POLR2H Rabbit pAb (CAB13339) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunoprecipitation analysis of 200 μg extracts of MCF7 cells using 1 μg POLR2H antibody (CAB13339). Western blot was performed from the immunoprecipitate using POLR2H antibody (CAB13339) at a dilution of 1:1000.
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