Metabolism Antibodies 1

PHGDH Rabbit Polyclonal Antibody (CAB10461)

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SKU:
CAB10461
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Reactivity:
Human, Mouse, Rat
Synonyms:
NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113, PHGDH
Research Use:
Metabolism
Frequently bought together:

Description

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PHGDH Antibody (CAB10461)

The PHGDH Antibody (CAB10461) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive to human samples, making it ideal for applications such as Western blotting.PHGDH is an essential enzyme involved in cancer metabolism, as it is often upregulated in cancer cells to support their rapid growth. By targeting PHGDH with this antibody, researchers can gain insights into the role of this enzyme in cancer progression and potentially identify new therapeutic targets.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PHGDH Antibody
SKU:CAB10461
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:EEPP RDRA LVDH ENVI SCPH LGAS TKEA QSRC GEEI AVQF VDMV KGKS LTGV VNAQ ALTS AFSP HTKP WIGL AEAL GTLM RAWA GSPK GTIQ VITQ GTSL KNAG NCLS PAVI VGLL KEAS KQAD VNLV NAKL LVKE AGLN VTTS HSPA APGE QGFG ECLL AVAL AGAP YQAV GLVQ GTTP VLQG LNGA VFRP EVPL RRDL PLLL FRTQ TSDP AMLP TMIG LLAE AGVR LLSY QTSL VSDG ETWH VMGI SSLL PSLE AWKQ HVTE AFQF HF
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:100 - 1:500
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113, PHGDH
Positive Sample:HeLa, HT-29, MCF7, NIH/3T3, C6, Mouse brain
Cellular Localization:Cytosol, Extracellular Exosome.
Calculated MW:57kDa
Observed MW:57kDa

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Purification MethodAffinity purification
Gene ID26227
RRIDAB_2758010
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PHGDH Rabbit pAb (CAB10461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates using PHGDH Rabbit pAb (CAB10461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageImmunofluorescence analysis of H9C2 cells using PHGDH Rabbit pAb (CAB10461) at dilution of 1:100. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of U2OS cells using PHGDH Rabbit pAb (CAB10461) at dilution of 1:100. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using PHGDH Rabbit pAb (CAB10461) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using PHGDH Rabbit pAb (CAB10461) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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