KO Validated Antibodies 1

[KO Validated] NDUFB4 Rabbit Polyclonal Antibody (CAB13820)

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SKU:
CAB13820
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
B15, CI-B15, B4
Research Use:
Cell Biology
Frequently bought together:

Description

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[KO Validated] NDUFB4 Antibody (CAB13820)

The [KO Validated] NDUFB4 Antibody (CAB13820) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blotting applications.NDUFB4 plays a key role in the electron transport chain and is essential for ATP synthesis in mitochondria. Dysregulation of NDUFB4 has been linked to various diseases, including neurodegenerative disorders and metabolic syndromes.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] NDUFB4 Antibody
SKU:CAB13820
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSFP KYKP SSLR TLPE TLDP AEYN ISPE TRRA QAER LAIR AQLK REYL LQYN DPNR RGLI ENPA LLRW AYAR TINV YPNF RPTP KNSL MG
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:B15, CI-B15, B4
Positive Sample:U-87MG, HeLa, 293T, Mouse heart, Mouse kidney, Mouse liver
Cellular Localization:Matrix Side, Mitochondrion Inner Membrane, Single-Pass Membrane Protein.
Calculated MW:15kDa
Observed MW:15kDa

This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.

Purification MethodAffinity purification
Gene ID4710
RRIDAB_2861696
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] NDUFB4 Rabbit pAb (CAB13820) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] NDUFB4 Rabbit pAb (CAB13820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat kidney using [KO Validated] NDUFB4 Rabbit pAb (CAB13820) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of L929 cells using [KO Validated] NDUFB4 Rabbit pAb (CAB13820) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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