Cell Cycle Antibodies 1

HEC1/NDC80 Rabbit Polyclonal Antibody (CAB5411)

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SKU:
CAB5411
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse
Synonyms:
HEC, HEC1, TID3, KNTC2, HsHec1, hsNDC80, HEC1/NDC80
Research Use:
Cell Cycle
Frequently bought together:

Description

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HEC1/NDC80 Antibody (CAB5411)

The HEC1/NDC80 Antibody (CAB5411) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications.The Ndc80 protein plays a crucial role in mitosis, ensuring accurate chromosome alignment and segregation. Dysregulation of Ndc80 has been linked to chromosomal instability and cancer development, highlighting its significance in cell division and genomic stability.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:HEC1/NDC80 Antibody
SKU:CAB5411
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ETIK QENT RLQN IIDN QKYS VADI ERIN HERN ELQQ TINK LTKD LEAE QQKL WNEE LKYA RGKE AIET QLAE YHKL ARKL KLIP KGAE NSKG YDFE IKFN PEAG ANCL VKYR AQVY VPLK ELLN ETEE EINK ALNK KMGL EDTL EQLN AMIT ESKR SVRT LKEE VQKL DDLY QQKI KEAE EEDE KCAS ELES LEKH KHLL ESTV NQGL SEAM NELD AVQR EYQL VVQT TTEE RRKV GNNL QRLL EMVA THVG SVEK HLEE QIAK VDRE YEEC MSED LSEN IKEI RDKY EKKA TLIK SSEE
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HEC, HEC1, TID3, KNTC2, HsHec1, hsNDC80, HEC1/NDC80
Positive Sample:K-562, Lovo, MCF7, 22Rv1, THP-1, 293T, Mouse testis
Cellular Localization:Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:74kDa
Observed MW:80kDa

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Purification MethodAffinity purification
Gene ID10403
RRIDAB_2766219
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HEC1/NDC80 Rabbit pAb (CAB5411) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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