Cell Biology Antibodies 8

NCF4 Rabbit Polyclonal Antibody (CAB2096)

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SKU:
CAB2096
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
NCF, CGD3, P40PHOX, SH3PXD4, NCF4/p40-phox
Research Use:
Cell Biology
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Description

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NCF4 Antibody (CAB2096)

The NCF4 Antibody (CAB2096) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been extensively validated for use in Western blot applications. By specifically binding to the NCF4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential reagent for investigations in immunology and cancer research.NCF4, also known as p40phox, is a critical player in the assembly of the phagocyte NADPH oxidase complex, which is essential for the production of reactive oxygen species involved in immune defense and inflammation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:NCF4 Antibody
SKU:CAB2096
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MAVA QQLR AESD FEQL PDDV AISA NIAD IEEK RGFT SHFV FVIE VKTK GGSK YLIY RRYR QFHA LQSK LEER FGPD SKSS ALAC TLPT LPAK VYVG VKQE IAEM RIPA LNAY MKSL LSLP VWVL MDED VRIF FYQS PYDS EQVP QALR RLRP RTRK VKSV SPQG NSVD RMAA PRAE ALFD FTGN SKLE LN
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NCF, CGD3, P40PHOX, SH3PXD4, NCF4/p40-phox
Positive Sample:U-937, RAW264.7, Rat lung
Cellular Localization:Cytoplasm, Cytoplasmic Side, Endosome Membrane, Membrane, Peripheral Membrane Protein, Cytosol.
Calculated MW:39kDa
Observed MW:40kDa

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification MethodAffinity purification
Gene ID4689
RRIDAB_2764115
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using NCF4/p40-phox Rabbit pAb (CAB2096) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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