NCF4 Rabbit Monoclonal Antibody (CAB0935)

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SKU:
CAB0935
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Synonyms:
NCF, CGD3, P40PHOX, SH3PXD4, NCF4/p40-phox
Research Use:
Cell Biology
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Description

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NCF4 Monoclonal Antibody (CAB0935)

The NCF4 Monoclonal Antibody (CAB0935) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is known for its high reactivity with human samples and is validated for use in Western blot applications.NCF4 is a key component of the phagocytic NADPH oxidase complex, essential for the production of reactive oxygen species in response to infections and inflammation. Dysregulation of NCF4 has been implicated in various inflammatory disorders, making it an important target for research in immunology and infectious diseases.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:NCF4 Monoclonal Antibody
SKU:CAB0935
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC2553
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LRCY YYED TIST IKDI AVEE DLSS TPLL KDLL ELTR REFQ REDI ALNY RDAE GDLV RLLS DEDV ALMV RQAR GLPS QKRL FPWK LHIT QKDN YRVY NTMP
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NCF, CGD3, P40PHOX, SH3PXD4, NCF4/p40-phox
Positive Sample:THP-1
Cellular Localization:Cytoplasm, Cytoplasmic Side, Endosome Membrane, Membrane, Peripheral Membrane Protein, Cytosol.
Calculated MW:39kDa
Observed MW:40kDa

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification MethodAffinity purification
Gene ID4689
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from THP-1 cells, using NCF4/p40-phox Rabbit mAb (CAB0935) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NCF4/p40-phox Rabbit mAb (CAB0935) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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