KO Validated Antibodies 1

[KO Validated] MAP2K4 Rabbit Polyclonal Antibody (CAB14781)

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SKU:
CAB14781
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Reactivity:
Human, Mouse, Rat
Synonyms:
JNKK, MEK4, MKK4, SEK1, SKK1, JNKK1, SERK1, MAPKK4, PRKMK4, SAPKK1, SAPKK-1, K4
Research Use:
Cell Death
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Description

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[KO Validated] MAP2K4 Antibody (CAB14781)

The [KO Validated] MAP2K4 Antibody (CAB14781) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.MAP2K4, also known as dual specificity mitogen-activated protein kinase kinase 4, is involved in the MAPK signaling pathway and is essential for the regulation of cell proliferation and survival.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] MAP2K4 Antibody
SKU:CAB14781
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LATG RFPY PKWN SVFD QLTQ VVKG DPPQ LSNS EERE FSPS FINF VNLC LTKD ESKR PKYK ELLK HPFI LMYE ERAV EVAC YVCK ILDQ MPAT PSSP MYVD
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:JNKK, MEK4, MKK4, SEK1, SKK1, JNKK1, SERK1, MAPKK4, PRKMK4, SAPKK1, SAPKK-1, K4
Positive Sample:SH-SY5Y, BT-474, MCF-7, mouse thymus, mouse liver, rat liver
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:44kDa
Observed MW:44kDa

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID6416
RRIDAB_2861707
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] MAP2K4 Rabbit pAb (CAB14781) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from wild type (WT) and MAP2K4 knockout (KO) HeLa cells, using [KO Validated] MAP2K4 Rabbit pAb (CAB14781) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageWestern blot analysis of various lysates, using MAP2K4 Rabbit pAb (CAB14781) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of lysates from HeLa cells, using MAP2K4 Rabbit pAb (CAB14781) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat ovary using MAP2K4 Rabbit pAb (CAB14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse kidney using MAP2K4 Rabbit pAb (CAB14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using MAP2K4 Rabbit pAb (CAB14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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