KO Validated Antibodies 1

[KO Validated] IKKgamma Rabbit Polyclonal Antibody (CAB12536)

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SKU:
CAB12536
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse
Synonyms:
IP, IP1, IP2, FIP3, IKKG, IPD2, NEMO, FIP-3, Fip3p, IMD33, SAIDX, AMCBX1, EDAID1, IKKAP1, ZC2HC9, IKK-gamma, Kγ
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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[KO Validated] IKKgamma Antibody (CAB12536)

The [KO Validated] IKKgamma Antibody (CAB12536) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has been validated for use in various applications, including Western blot and immunohistochemistry.The IKBKG protein plays a central role in the activation of NF-kappa-B, a transcription factor that regulates genes involved in inflammation, immunity, cell proliferation, and apoptosis. Dysregulation of the NF-kappa-B pathway has been implicated in various diseases, including cancer, inflammatory disorders, and autoimmune conditions.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] IKKgamma Antibody
SKU:CAB12536
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MNRH LWKS QLCE MVQP SGGP AADQ DVLG EESP LGKP AMLH LPSE QGAP ETLQ RCLE ENQE LRDA IRQS NQIL RERC EELL HFQA SQRE EKEF LMCK FQEA RKLV ERLG LEKL DLKR QKEQ ALRE VEHL KRCQ QQMA EDKA SVKA QVTS LLGE LQES QSRL EAAT KECQ ALEG RARA ASEQ ARQL ESER EALQ QQHS VQVD QLRM QGQS VEAA LRME RQAA SEEK RKLA QLQV AYHQ LFQE YDNH IKSS VVGS ERKR GMQL EDLK QQLQ QAEE ALVA KQEV IDKL KEEA EQHK IVME TVPV LKAQ ADIY KADF QAER QARE KLAE KKEL LQEQ LEQL QREY SKLK ASCQ ESAR IEDM RKRH VEVS QAPL PPAP AYLS SPLA LPSQ RRSP PEEP PDFC CPKC QYQA PDMD TLQI HVME CIE
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:IP, IP1, IP2, FIP3, IKKG, IPD2, NEMO, FIP-3, Fip3p, IMD33, SAIDX, AMCBX1, EDAID1, IKKAP1, ZC2HC9, IKK-gamma, Kγ
Positive Sample:A-549, Mouse liver
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:48kDa/56kDa/37kDa
Observed MW:48kDa

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Purification MethodAffinity purification
Gene ID8517
RRIDAB_2861670
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] IKKγ Rabbit pAb (CAB12536) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageWestern blot analysis of lysates from wild type (WT) and IKKγ knockout (KO) 293T cells, using [KO Validated] IKKγ Rabbit pAb (CAB12536) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
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