Cell Biology Antibodies 1

GDF15 Rabbit Polyclonal Antibody (CAB0185)

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SKU:
CAB0185
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, GDF15
Research Use:
Cell Biology
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Description

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GDF15 Antibody (CAB0185)

The GDF15 Antibody (CAB0185) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and is optimized for Western blot applications.GDF15 is a key biomarker for various diseases, including cancer, cardiovascular disease, and metabolic disorders. Its involvement in regulating cell growth, apoptosis, and energy balance makes it a target of interest for researchers seeking to understand disease mechanisms and develop new therapeutic strategies.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:GDF15 Antibody
SKU:CAB0185
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:EDSR FREL RKRY EDLL TRLR ANQS WEDS NTDL VPAP AVRI LTPE VRLG SGGH LHLR ISRA ALPE GLPE ASRL HRAL FRLS PTAS RSWD VTRP LRRQ LSLA RPQA PALH LRLS PPPS QSDQ LLAE SSSA RPQL ELHL RPQA ARGR RRAR ARNG DHCP LGPG RCCR LHTV RASL EDLG WADW VLSP REVQ VTMC IGAC PSQF RAAN MHAQ IKTS LHRL KPDT VPAP CCVP ASYN PMVL IQKT DTGV SLQT YDDL LAKD CHCI
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, GDF15
Positive Sample:PC-3, HT-1080
Cellular Localization:Secreted.
Calculated MW:34kDa
Observed MW:35kDa

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress.

Purification MethodAffinity purification
Gene ID9518
RRIDAB_2757008
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using GDF15 Rabbit pAb (CAB0185) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using GDF15 Rabbit pAb (CAB0185) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunoprecipitation analysis of 200 μg extracts of HT-1080 cells using 3 μg GDF15 antibody (CAB0185). Western blot was performed from the immunoprecipitate using GDF15 antibody (CAB0185) at a dilution of 1:1000.
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