Signal Transduction Antibodies 3

DBI Rabbit Polyclonal Antibody (CAB5370)

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SKU:
CAB5370
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Synonyms:
EP, ACBP, ACBD1, CCK-RP, DBI
Research Use:
Signal Transduction
Frequently bought together:

Description

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DBI Antibody (CAB5370)

The DBI Antibody (CAB5370) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has high specificity for human samples, making it ideal for use in Western blot applications to detect and analyze DBI expression in different cell types.DBI, also known as diazepam-binding inhibitor, is a multifunctional protein that interacts with various molecules, including benzodiazepines, to modulate their effects on neurotransmission and cell signaling. Its involvement in stress and anxiety regulation, as well as its potential role in neurological disorders, makes it an intriguing target for further research in neuroscience and pharmacology.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:DBI Antibody
SKU:CAB5370
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MWGD LWLL PPAS ANPG TGTE AEFE KAAE EVRH LKTK PSDE EMLF IYGH YKQA TVGD INTE RPGM LDFT GKAK WDAW NELK GTSK EDAM KAYI NKVE ELKK KYGI
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EP, ACBP, ACBD1, CCK-RP, DBI
Positive Sample:HeLa, HepG2, U-87MG, 293T
Cellular Localization:Endoplasmic Reticulum, Golgi Apparatus.
Calculated MW:10-12kDa
Observed MW:12kDa

This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

Purification MethodAffinity purification
Gene ID1622
RRIDAB_2766180
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and DBI knockout (KO) 293T cells, using [KO Validated] DBI Rabbit pAb (CAB5370) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates, using DBI Rabbit pAb (CAB5370) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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