Signal Transduction Antibodies 1

DBI Rabbit Polyclonal Antibody (CAB13293)

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SKU:
CAB13293
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
EP, ACBP, ACBD1, CCK-RP, DBI
Research Use:
Signal Transduction
Frequently bought together:

Description

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DBI Antibody (CAB13293)

The DBI Antibody (CAB13293) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is suitable for use in Western blot applications. By binding to the DBI protein, researchers can accurately detect and analyze this target in a variety of cell types, making it an excellent choice for studies in cell biology and neurobiology.DBI, also known as diazepam binding inhibitor, plays a crucial role in the regulation of GABAergic neurotransmission and has been implicated in processes such as anxiety, sleep, and memory.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:DBI Antibody
SKU:CAB13293
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MWGD LWLL PPAS ANPG TGTE AEFE KAAE EVRH LKTK PSDE EMLF IYGH YKQA TVGD INTE RPGM LDFT GKAK WDAW NELK GTSK EDAM KAYI NKVE ELKK KYGI
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EP, ACBP, ACBD1, CCK-RP, DBI
Positive Sample:HeLa, HepG2, U-87MG, Rat testis, Rat ovary
Cellular Localization:Endoplasmic Reticulum, Golgi Apparatus.
Calculated MW:10-12kDa
Observed MW:12kDa

This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.

Purification MethodAffinity purification
Gene ID1622
RRIDAB_2760152
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using DBI Rabbit pAb (CAB13293) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageWestern blot analysis of various lysates using DBI Rabbit pAb (CAB13293) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using DBI Rabbit pAb (CAB13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using DBI Rabbit pAb (CAB13293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (CAB13293). Western blot was performed from the immunoprecipitate using DBI antibody (CAB13293) at a dilution of 1:1000.
Product ImageImmunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg DBI antibody (CAB13293). Western blot was performed from the immunoprecipitate using DBI antibody (CAB13293) at a dilution of 1:1000.
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