Cell Biology Antibodies 7

COL4A3 Rabbit Polyclonal Antibody (CAB16359)

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SKU:
CAB16359
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
ATS2, ATS3, BFH2, COL4A3
Research Use:
Cell Biology
Frequently bought together:

Description

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COL4A3 Antibody (CAB16359)

The COL4A3 Antibody (CAB16359) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the COL4A3 protein, enabling precise detection and analysis in different cell types.Collagen IV alpha-3 is essential for maintaining the structural integrity of basement membranes and plays a crucial role in cell adhesion, migration, and tissue organization.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:COL4A3 Antibody
SKU:CAB16359
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:AIAI AVHS QTTD IPPC PHGW ISLW KGFS FIMF TSAG SEGT GQAL ASPG SCLE EFRA SPFL ECHG RGTC NYYS NSYS FWLA SLNP ERMF RKPI PSTV KAGE LEKI ISRC QVCM KKR
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ATS2, ATS3, BFH2, COL4A3
Positive Sample:Mouse kidney
Cellular Localization:Secreted, Basement Membrane, Extracellular Matrix, Extracellular Space.
Calculated MW:162kDa
Observed MW:162kDa/26kDa

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

Purification MethodAffinity purification
Gene ID1285
RRIDAB_2768994
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse kidney, using COL4A3 Rabbit pAb (CAB16359) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunofluorescence analysis of HeLa cells using COL4A3 Rabbit pAb (CAB16359) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using COL4A3 Rabbit pAb (CAB16359) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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